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the Cell Tracker Green was diluted 1: 1000 to 1 × Assay Buffer and the dose was expanded accordingly for large sample size determination
IL-6 in normal human blood is in the range of 1 pg/mL
Whereas Sα-smooth muscle expression is normally restricted to smooth muscle cells
so that fluorescein is stable and covalently bound on the sample
Each component of the kit was left to equilibrate at room temperature for 30 minutes before operation was started
Human IL-17A, His tag Secondary Antibody the Cell Tracker Green wasProduct Specification Species Human Synonyms Cytotoxic T lymphocyte associated antigen 8 (CTLA 8) Accession Q16552 Amino Acid Sequence Protein sequence(Q16552, Gly24 Ala155, with C 10*His) GITIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHVAGGGGSHHHHHHHHHH Expression System HEK293 Molecular Weight Theoretical: 16. 8kDa Actual: 17, 20kDa Purity 95% by SDS PAGE Endotoxin
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