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Long-chain acyl-CoA synthetase 2 (LACS 2)
and HRP enzyme conjugate are sequentially added to microwells pre-coated with a matrix metalloproteinase 2 (MMP-2) capture antibody
Due to the 3´→5´ exonuclease activity of the enzyme
which cleave their substrates at the C-terminal aspartate residue
they are separated by an intermediate amino acid
Chicken LZM ELISA Kit Proteins and Enzymes Long-chain acyl-CoA synthetase 2 (LACSProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20