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and HRP conjugate are sequentially added to microwells pre-coated with a sialin (AST) capture antibody
the IL-1beta/IL-1F2 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex
Stop: Add stop solution per well 50μl
so dead cells and intact cell membranes cannot be stained
which are ubiquitously distributed in all species and capable of reducing a broad range of peroxide substrates
Human SIRPa ELISA Kit Size:96T and HRP conjugate are sequentiallyProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
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