FireGene Universal Plant Nuclei Isolation Kit for Single-Nucleus Sequencing DNA Extraction when resuspending the cell pellet

when resuspending the cell pellet with RPMI 1640 containing 2% FBS

A: The loose cardiomyocyte pellet is because the cell surface is negatively charged

minimal manual operating steps

but an additional PBS washing step is required to avoid residual lysis buffer affecting subsequent experiments (e

and the hair cell recovery rate is more than 40% higher than that of FG-BA3303

FireGene Universal Plant Nuclei Isolation Kit for Single-Nucleus Sequencing DNA Extraction when resuspending the cell pelletFireGene Universal Plant Nuclei Isolation Kit is designed for the isolation and extraction of cell nuclei from cryopreserved tissue samples of higher plants. The kit uses surfactants to release nuclei, remove chloroplasts, and maintain nuclear membrane stability. After filtration, centrifugation, density layer separation, and chloroplast removal, the workflow generates clean single nucleus suspensions suitable for single nucleus transcriptome