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" Molecular Therapy Nucleic Acids 12 (2018): 530-542
a dimethylformamidyl (dmf) base-protecting group on guanine
where m1Ψ provides stronger suppression of innate immune responses and supports robust protein expression in vivo
Delivers high coupling efficiency and consistently strong crude yields
and Robert G
DMT-2'-F-dG(dmf)-CE Phosphoramidite IVT " Molecular Therapy Nucleic AcidsDMT 2 F dG(dmf) CE Phosphoramidite is a 2' fluoro (2' F) phosphoramidite monomer used for solid phase oligonucleotide synthesis. It contains a 2' F modification on the sugar of deoxyguanosine (dG) with dimethylformamidyl (dmf) base protection, a 5 dimethoxytrityl (DMT) group for 5' hydroxyl protection, and a cyanoethyl (CE) protecting group on phosphite. Synthesis of RNA oligonucleotides using 2' F phosphoramidite increases the nuclease resistance and
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