1-N-Me-Pseudo UTP, 100mM Solution Application_mRNA } The 10mM GTP is

} The 10mM GTP is a buffer solution used in conjunction with the Vaccinia Capping Enzyme

[3] Burmeister

Pfundheller

Template DNA

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1-N-Me-Pseudo UTP, 100mM Solution Application_mRNA } The 10mM GTP isN1 methyl pseudouridine 5 triphosphate (m1TP) is a chemically modified uridine nucleotide designed for enzymatic incorporation into RNA during IVT using RNA polymerases such as T7 RNA polymerase. m1TP is an N1 methylated derivative of pseudouridine that preserves base pairing properties while altering innate immune recognition. When substituted for uridine in IVT, m1 reduces activation of innate immune sensors and enhances translational efficiency of