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The framed area was magnified (20x) in the right to show the branches and dendritic spines of a pyramidal neuron located in layer III
(1) Tissue Handling: Animals are intracardially perfused with saline followed by a fixative (Catalog Number: 003780) developed by Bioenno Tech
The section was counterstained with Bioenno Cresyl Violet (Cat # 003003)
· How to apply the sliceGolgi kit on organotypic slice cultures
the staining of neurons can be performed on either free-floating or mounted sections (50~250 µm thickness)
Fixative Kit (Cat#003780) Slide Mount The framed area was magnified(Distributor and quantity discounts available) Please send an email to contact@bioenno. com to order. For common questions and answers, please check our FAQ The sliceGolgi Kit is designed for running Golgi staining on slices sections. The impregnation and staining can be performed on free floating sections (50~400 micron thickness). The additional Fixative Kit uses Bioennos aldehyde to process additional samples. The kit has been extensively tested